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<t>MOLT-4</t> <t>chemotactic</t> response towards CCL25 is attenuated by VUP binding. ( A ) Percentage of migrated cells towards CCL25 across a titration of chemokine concentrations. ( B ) Percentage of migrated cells towards a fixed concentration of CCL25 (260 nM, corresponding to the highest migration measured in (A)) preincubated with miniproteins (10 µM). Values represent the mean ± SD of three independent experiments measured in technical triplicates by flow cytometry. Significance was determined using Welch ANOVA followed by a Dunnet’s T3 multiple comparisons test comparing to no VUP. *p<0.05.
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<t>MOLT-4</t> <t>chemotactic</t> response towards CCL25 is attenuated by VUP binding. ( A ) Percentage of migrated cells towards CCL25 across a titration of chemokine concentrations. ( B ) Percentage of migrated cells towards a fixed concentration of CCL25 (260 nM, corresponding to the highest migration measured in (A)) preincubated with miniproteins (10 µM). Values represent the mean ± SD of three independent experiments measured in technical triplicates by flow cytometry. Significance was determined using Welch ANOVA followed by a Dunnet’s T3 multiple comparisons test comparing to no VUP. *p<0.05.
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MOLT-4 chemotactic response towards CCL25 is attenuated by VUP binding. ( A ) Percentage of migrated cells towards CCL25 across a titration of chemokine concentrations. ( B ) Percentage of migrated cells towards a fixed concentration of CCL25 (260 nM, corresponding to the highest migration measured in (A)) preincubated with miniproteins (10 µM). Values represent the mean ± SD of three independent experiments measured in technical triplicates by flow cytometry. Significance was determined using Welch ANOVA followed by a Dunnet’s T3 multiple comparisons test comparing to no VUP. *p<0.05.

Journal: bioRxiv

Article Title: CCL25 targeting by de novo protein binders differentially suppress CCR9 and ACKR4 activation and transform CCL25 into a biased agonist

doi: 10.64898/2026.01.23.701245

Figure Lengend Snippet: MOLT-4 chemotactic response towards CCL25 is attenuated by VUP binding. ( A ) Percentage of migrated cells towards CCL25 across a titration of chemokine concentrations. ( B ) Percentage of migrated cells towards a fixed concentration of CCL25 (260 nM, corresponding to the highest migration measured in (A)) preincubated with miniproteins (10 µM). Values represent the mean ± SD of three independent experiments measured in technical triplicates by flow cytometry. Significance was determined using Welch ANOVA followed by a Dunnet’s T3 multiple comparisons test comparing to no VUP. *p<0.05.

Article Snippet: MOLT-4 cells (ATCC) were collected by centrifugation at 125 x g for 5 min and subsequently resuspended in RPMI 1640 + 10% FBS (Gibco) at a density of 400k/ml.

Techniques: Binding Assay, Titration, Concentration Assay, Migration, Flow Cytometry